Unknown and Undiluted Titration Date Essay Example | Topics and Well Written Essays - 500 words - 6

Unknown and Undiluted Titration Date - Essay Example Furthermore, the concentration of Cca2+ is again subtracted from all the total concentration, in addition, the Mg2+ concentration is 0.0227+-0.005M after calculation. After Na2H2EDTA.2H2O is prepared and goes in contamination by water in 0.3 percentage the standard solution has to be corrected. Here below are the equations that analyses and further determines the EDTA solution concentration. Mass without impurity is the mass corrected and the mass actual is the dried chelating agent; EDTA concentration bears the name as CEDTA; the initial MW represents molecular weight, and the volume of the solution (500 ml) is V solution. Propagation of the errors calculates the uncertainty. The balance of uncertainty is +- 0.0001g and that of volumetric flask’s uncertainty is +-0.15 ml. the standard relative deviation is initialized by SRD. Calculation of results is in tabulation in the table. Results show that both relative derivation volume and relative derivation balance is +-0.0003.Moreso, after calculating using the values above EDTA concentration solution is 0.0018+-0.0004M. 1.00ml Ca/Mg solution in the spike is contained in the blank titration, Ph 10 buffer solution, water in conjunction with the indicator. From all the titration, the data in trial one from all the titrations undergoes nullification due to it being carried out hastily. Additional of 1.00ml in dilution of the unknown sample to the blank titration is put in a performance. The equation below is used to calculate V total: Both the standard deviation and the mean of part one and part two titrations are as follows and in illustration in table 4.Concentration of Mg2+ and Ca2+ can be identified from the values in the unknown sample.  

Genetic Lab Report Example | Topics and Well Written Essays - 1500 words

Genetic - Lab Report Example The effects of other processes, such as genetic imprinting, are important in determining the traits for other characteristics of the organism. Sequencing the genetic material of a species, and determining all coding sequences and their corresponding proteins are vital projects in the field of molecular biology. The human genome project had been successful in sequencing the DNA of humans (Venter et al., 2001). The studies of (Kyrylkova et al., 2012; Yu et al., 2012) determine the characteristics corresponding to a particular genetic sequence by preventing the transcription of the latter and observing the changes afterward. A sequence identified to determine a particular characteristic can also be used as a molecular marker to determine the presence or absence of trait, even without actually seeing it in the organism. For example, markers for sex can help distinguish between males and females in monomorphic species or their young. The objective of this experiment was to demonstrate the use of DNA samples to determine the characteristics of the organism, particularly its sex. Specifically, the activity aimed to isolate DNA from different sources, blood, muscle and feather. After purification, the DNA isolates underwent polymerase chain reaction (PCR) using sexing primers 2250F and 2718R to replicate the sequence for sex determination. Agarose gel electrophoresis of the PCR products were compared with that of known male and female samples to identify the sex of the chicken from which the samples were obtained. Qiagen DNeasy Blood and Tissue Extraction Kit was used for extraction. Briefly, the tissues sample was lysed by incubating it in a solution containing 20 Â µl proteinase K, 4 Â µl RNAse A and 166 Â µl phosphate buffer solution (PBS) (blood) or 180 Â µl Buffer ATL (muscle or feather) for 30 min. After mixing with 200 Â µl Buffer AL for 15 sec, the DNA was precipitated by addition of 200 Â µl 95%